- \n
- CRISPR\/Cas technology, RNA biology, Immunology, Cancer biology, Protein expression<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1535953408795{margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text]<\/p>\n
Research<\/h1>\n
[\/vc_column_text][vc_column_text]<\/p>\n
\n\n- \n
- Final Year Research project:<\/em><\/strong> Effect of CRISPR\/Cas9 KO of pfk2 on oxygen consumption rate in human HepG2 cells; Throughout the project, appropriate genomic targets for CRISPR\/Cas9 were discovered in pfk2 and target specific CRISPR\/Cas9 components were designed with maximum accuracy and efficacy to achieve KO of pfk2. The intention was to exploit the CRISPR-Cas9 system to achieve successful KO of pfk2 via mutation induction and HDR and thereby to investigate the effect of CRISPR\/Cas9 mediated modification of pfk2 on uncontrolled cellular proliferation in HepG2 cells. The ultimate goal was to investigate the propriety of pfk2 as a therapeutic target for hepatocellular carcinoma.Supervisors:<\/em><\/strong> Prof. R. S. Dassanayake, Dr. Gayathri Silva.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1535953408795{margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text]<\/p>\n
Publications<\/h1>\n
[\/vc_column_text][vc_column_text]<\/p>\n
\n\n- \n
- Review article on Correction of beta thalassemia in Asia using CRISPR\/Cas9 technology.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"
[vc_row][vc_column width=”1\/4″][vc_single_image image=”243″ img_size=”large” style=”vc_box_shadow_border”][\/vc_column][vc_column width=”3\/4″][vc_column_text] Temporary Assistant lecturer (2018-2019). Department of Chemistry University of Colombo Colombo 00300 Sri Lanka […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-pagebuilder.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/136"}],"collection":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/comments?post=136"}],"version-history":[{"count":4,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/136\/revisions"}],"predecessor-version":[{"id":244,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/136\/revisions\/244"}],"wp:attachment":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/media?parent=136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Review article on Correction of beta thalassemia in Asia using CRISPR\/Cas9 technology.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
- Final Year Research project:<\/em><\/strong> Effect of CRISPR\/Cas9 KO of pfk2 on oxygen consumption rate in human HepG2 cells; Throughout the project, appropriate genomic targets for CRISPR\/Cas9 were discovered in pfk2 and target specific CRISPR\/Cas9 components were designed with maximum accuracy and efficacy to achieve KO of pfk2. The intention was to exploit the CRISPR-Cas9 system to achieve successful KO of pfk2 via mutation induction and HDR and thereby to investigate the effect of CRISPR\/Cas9 mediated modification of pfk2 on uncontrolled cellular proliferation in HepG2 cells. The ultimate goal was to investigate the propriety of pfk2 as a therapeutic target for hepatocellular carcinoma.Supervisors:<\/em><\/strong> Prof. R. S. Dassanayake, Dr. Gayathri Silva.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n