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- Transgenic technology, Cancer Biology<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1535953408795{margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text]<\/p>\n
Research<\/h1>\n
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- Final Year Research project:<\/em><\/strong> Identification of an ideal microorganism (Bacterial) and development of a novel micro-biosensor using transgenic techniques to detect the metal ion (Cadmium) contamination in water; This research project is a novel approach in Sri Lanka as a reliable, cost effective method for detection of Cd2+<\/sup> in the environment, especially in drinking water. The main reason for this is, Chronic renal failure (CRF) is one of major health issue which associated with environmental contamination by heavy metals such as cadmium. In order to achieve this purpose cadmium sensing novel DNA cassette based on cadmium resistance mechanism in Pseudomonas aeruginosa PA7 (cadA\/cadR operon system) was transformed into E. coli <\/em>BL21.\u00a0\u00a0 In here, cadA promoter fused to egfp (enhanced green fluorescent protein) and T7 promoter fused with cadR. Eventually, it was able to develop genetically engineered whole-cell bacterial biosensor to produce green color signals with higher efficiency by expressing green fluorescent protein as a response to cadmium in water.Supervisors:<\/em><\/strong> Prof. R.S. Dassanayake, Prof. Y.I.N.S. Gunawardena, Prof. Nalin De Silva, Dr. Rohini De Silva<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"
[vc_row][vc_column width=”1\/4″][vc_single_image image=”255″ img_size=”large” style=”vc_box_shadow_border”][\/vc_column][vc_column width=”3\/4″][vc_column_text] Temporary Demonstrator (2018-2019). Department of Chemistry University of Colombo Colombo 00300 Sri Lanka Email: […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-pagebuilder.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/142"}],"collection":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/comments?post=142"}],"version-history":[{"count":3,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/142\/revisions"}],"predecessor-version":[{"id":257,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/142\/revisions\/257"}],"wp:attachment":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/media?parent=142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Final Year Research project:<\/em><\/strong> Identification of an ideal microorganism (Bacterial) and development of a novel micro-biosensor using transgenic techniques to detect the metal ion (Cadmium) contamination in water; This research project is a novel approach in Sri Lanka as a reliable, cost effective method for detection of Cd2+<\/sup> in the environment, especially in drinking water. The main reason for this is, Chronic renal failure (CRF) is one of major health issue which associated with environmental contamination by heavy metals such as cadmium. In order to achieve this purpose cadmium sensing novel DNA cassette based on cadmium resistance mechanism in Pseudomonas aeruginosa PA7 (cadA\/cadR operon system) was transformed into E. coli <\/em>BL21.\u00a0\u00a0 In here, cadA promoter fused to egfp (enhanced green fluorescent protein) and T7 promoter fused with cadR. Eventually, it was able to develop genetically engineered whole-cell bacterial biosensor to produce green color signals with higher efficiency by expressing green fluorescent protein as a response to cadmium in water.Supervisors:<\/em><\/strong> Prof. R.S. Dassanayake, Prof. Y.I.N.S. Gunawardena, Prof. Nalin De Silva, Dr. Rohini De Silva<\/li>\n<\/ul>\n<\/div>\n<\/div>\n