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- CRISPR\/Cas technology, Immunology, Cancer biology, gene therapy<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1535953408795{margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text]<\/p>\n
Research<\/h1>\n
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- Final year Research Project<\/strong>: Effect of CRISPR\/Cas9 KO of pfk1 on Oxygen consumption rates in mammalian cell. MCF-7 human cancer cell line was selected as the target cells and appropriate genomic targets were discovered for human pfk1 gene with bioinformatics tools. The ultimate goal of the project was to modify the pfk1 gene via CRISPR\/Cas9 genome editing tool and to investigate the effect of the said modification on the oxygen consumption rates of MCF -7 cells. Cell culturing techniques were utilized during the project to maintain a healthy MCF-7 cell line and Escherichia coli TOP10 cells were used for the CRISPR\/Cas9 vector propagation.Supervisors<\/strong>: Prof. R.S. Dassanayake, Dr. Gayathri Silva, Prof.Y.I.N.S. Gunawardene<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"
[vc_row][vc_column width=”1\/4″][vc_single_image image=”274″ img_size=”large” style=”vc_box_shadow_border”][\/vc_column][vc_column width=”3\/4″][vc_column_text] Temporary Academic Staff (2019). Department of Chemistry University of Colombo Colombo 00300 Sri Lanka […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-pagebuilder.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/147"}],"collection":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/comments?post=147"}],"version-history":[{"count":3,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/147\/revisions"}],"predecessor-version":[{"id":277,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/147\/revisions\/277"}],"wp:attachment":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/media?parent=147"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Final year Research Project<\/strong>: Effect of CRISPR\/Cas9 KO of pfk1 on Oxygen consumption rates in mammalian cell. MCF-7 human cancer cell line was selected as the target cells and appropriate genomic targets were discovered for human pfk1 gene with bioinformatics tools. The ultimate goal of the project was to modify the pfk1 gene via CRISPR\/Cas9 genome editing tool and to investigate the effect of the said modification on the oxygen consumption rates of MCF -7 cells. Cell culturing techniques were utilized during the project to maintain a healthy MCF-7 cell line and Escherichia coli TOP10 cells were used for the CRISPR\/Cas9 vector propagation.Supervisors<\/strong>: Prof. R.S. Dassanayake, Dr. Gayathri Silva, Prof.Y.I.N.S. Gunawardene<\/li>\n<\/ul>\n<\/div>\n<\/div>\n