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- RNA biology, Gene therapy, Immunology, Cancer Biology.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1535953408795{margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text]<\/p>\n
Research<\/h1>\n
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- Final Year Research project<\/strong>: Development of riboswitches mediated biosensor to detect fluoride levels in drinking water; This research is designed with the aim of determining F \u2013 content of water samples with the aid of naturally occurring bacterial riboswitches. The research work was carried out with the use of E.coli BW25113 cells transformed with recombinant vector pRS414 carrying either crcB knockout fluoride responsive riboswitch or WT fluoride responsive riboswitch from Pseudomonas syringae. Assay was carried out with the use of X-gal which get converted to a blue coloured compound (5,5\u2019-dibromo-4,4\u2019-dichloro-indigo) with the presence of F \u2013 that result in the expression of the downstream gene lacZ. Absorbance and fluorescence measurements of 5,5\u2019-dibromo-4,4\u2019-dichloro-indigo formed was used to qualitatively determine the groundwater F \u2013 content.Supervisors<\/strong>: Dr. Gayathri N. Silva, Prof. R. S. Dassanayake.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"
[vc_row][vc_column width=”1\/4″][vc_single_image image=”274″ img_size=”large” style=”vc_box_shadow_border”][\/vc_column][vc_column width=”3\/4″][vc_column_text] Temporary Academic Staff Department of Chemistry University of Colombo Colombo 00300 Sri Lanka Email: […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-pagebuilder.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/159"}],"collection":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/comments?post=159"}],"version-history":[{"count":3,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/159\/revisions"}],"predecessor-version":[{"id":279,"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/pages\/159\/revisions\/279"}],"wp:attachment":[{"href":"https:\/\/www.res.cmb.ac.lk\/chemistry\/btec\/wp-json\/wp\/v2\/media?parent=159"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Final Year Research project<\/strong>: Development of riboswitches mediated biosensor to detect fluoride levels in drinking water; This research is designed with the aim of determining F \u2013 content of water samples with the aid of naturally occurring bacterial riboswitches. The research work was carried out with the use of E.coli BW25113 cells transformed with recombinant vector pRS414 carrying either crcB knockout fluoride responsive riboswitch or WT fluoride responsive riboswitch from Pseudomonas syringae. Assay was carried out with the use of X-gal which get converted to a blue coloured compound (5,5\u2019-dibromo-4,4\u2019-dichloro-indigo) with the presence of F \u2013 that result in the expression of the downstream gene lacZ. Absorbance and fluorescence measurements of 5,5\u2019-dibromo-4,4\u2019-dichloro-indigo formed was used to qualitatively determine the groundwater F \u2013 content.Supervisors<\/strong>: Dr. Gayathri N. Silva, Prof. R. S. Dassanayake.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n