Miss. Dhanushika Thavakumaran

Temporary Assistant lecturer (2018-2019).

Department of Chemistry
University of Colombo
Colombo 00300
Sri Lanka

Email: otjayasinghe@gmail.com

Academic & Professional Qualifications

  • BSc

    B. Sc in and Biochemistry & Molecular biology (Special)

    Faculty of science, University of Colombo

    Research Interest

    • CRISPR/Cas technology, RNA biology, Immunology, Cancer biology, Protein expression

    Research

    • Final Year Research project: Effect of CRISPR/Cas9 KO of pfk2 on oxygen consumption rate in human HepG2 cells; Throughout the project, appropriate genomic targets for CRISPR/Cas9 were discovered in pfk2 and target specific CRISPR/Cas9 components were designed with maximum accuracy and efficacy to achieve KO of pfk2. The intention was to exploit the CRISPR-Cas9 system to achieve successful KO of pfk2 via mutation induction and HDR and thereby to investigate the effect of CRISPR/Cas9 mediated modification of pfk2 on uncontrolled cellular proliferation in HepG2 cells. The ultimate goal was to investigate the propriety of pfk2 as a therapeutic target for hepatocellular carcinoma.Supervisors: Prof. R. S. Dassanayake, Dr. Gayathri Silva.

    Publications

    • Review article on Correction of beta thalassemia in Asia using CRISPR/Cas9 technology.