Catharanthus roseus, valued for both ornamental value and medicinal properties, shows substantial genetic variation when growing propagated from seeds. This leads to wide differences in alkaloid content and agronomic traits among plants. Vegetative propagation is important for transferring genetic characteristics to the next generation without inducing variability; therefore, it plays a key role in the plant breeding program of C. roseus. The aim of the present is to develop an effective vegetative propagation protocol for C. roseus. The experiment was conducted using a two-factor factorial completely randomized design incorporating three types of stem cuttings of C. roseus native plants (softwood, semi-hardwood, and hardwood) and five growing media (sand, coir dust, 1:1 volume basis mixture of sand:coir dust, sand:compost, and sand:topsoil). Prior to planting, stem cuttings were treated with Captan 50% w/w fungicide and 0.3% Indole Butyric Acid powder. All cuttings were maintained in a mist-propagator environment under 50% shade condition. Data were recorded in the fifth week after the propagator opened on survival rate, number and length of newly produced shoots, number of roots, and fresh root weight. The results indicated that there were an interaction effect of growing media and the cutting types on root number and the root fresh weight. Accordingly, tip cuttings of C. roseus grown in sand alone medium demonstrated superior rooting performance, producing the highest mean number of roots and the greatest fresh root biomass during the experimental period. In contrast, semi-hardwood cuttings exhibited significantly the highest numbers of newly produced shoots per cutting. Tip cuttings had a 100% survival rate, demonstrating significantly higher success. This study demonstrated the high potential of stem cutting propagation in C. roseus, enabling effective plant multiplication within a relatively short period.