Cell and Molecular biology techniques
DNA and RNA isolations, PCR, electrophoresis, SSCP, RFLP, western-blot, slot-blot, RT-PCR, real-time PCR, PCR arrays, DNA sequencing, flow-cytometry, light microscopy, fluorescence microscopy, ELISA, immunohistochemistry and transcriptome analysis.
Cell culture experience and cell based assays
Handling and maintenance of a range of human cancer cell lines (MCF-7, MDA-MB-231, HepG2, SKOV-3, MRC-5, MCF-10A, NTERA-2, AN3CA, ACHN, HEK-292, NCI-H292, CaCo-2, Hs57T, SKBR-3, PC-3, LN-229 and A-431), isolation and culturing of cancer stem cells, development of drug resistant cancer cell lines, cytotoxic assays, assays to evaluate apoptosis, cell migration assays, oxidative stress marker assays, ChIP assays, cellular senescence assays, luciferase assays, cellular metabolism assays.
Computer skills
Operating Systems: All current Windows Systems Software / Applications: Current Microsoft Office Packages, Adobe Photoshop, Adobe Illustrator, Corel Draw Pro, Publisher, Turbo Tax, Quicken, QuickBooks, QuickBooksPro and Reference Manager.
Other skills
HPLC, TLC, GC/MS techniques, HPLC-MS, HPLC-DAD, interpretation of NMR and Mass spectroscopic data and structure elucidation of compounds.
Bioinformatics software
SwissDock, Swiss similarity, AutoDock Vina, PyRx, primer designing tools, ORF Finder, BioEdit, etc.