Tropical Medicine Research Center (TMRC) Project – Achievements from 2011-2017

Tropical Medicine Research Center (TMRC) Project – Achievements from 2011-2017

KEY SCIENTIFIC FINDINGS OF PUBLIC HEALTH SIGNIFICANCE

With the recognition of a leishmaniasis outbreak in 2001 a series of studies that followed enabled in-depth investigation of CL patients using clinical, biological and latest molecular techniques. Key scientific findings of public health significance achieved are as follows:

PUBLICATIONS (2001-2018)

  • Karunaweera ND, Ferreira MU. (2018). Leishmaniasis: current challenges and prospects for elimination with special focus on the South Asian region. Parasitology. 12:1-5. https://doi.org/10.1017/S0031182018000471
  • Karunaweera ND (2016). Leishmaniasis: Path towards elimination from the Indian subcontinent. Tropical Parasitology. 6(1): 2-4.
  • Siriwardana HVYD, Chandrawansa PH, Sirimanna G, Karunaweera ND (2012). Leishmaniasis in Sri Lanka: a decade old story (2002-2012). Sri Lanka Journal of Infectious Diseases, 2(2): 2-12. http://www.sljol.info/index.php/SLJID/issue/view/425
  • Karunaweera ND (2009). Leishmania donovani causing cutaneous leishmaniasis in Sri Lanka: A wolf in sheep’s clothing? Trends in Parasitology, 25(10):458-463.
  • Karunaweera ND, Rajapaksa U. (2009). Is Leishmaniasis in Sri Lanka benign and be ignored? Journal of Vector Borne Diseases, 46, 13-17.
  • Karunaweera ND. Leishmaniasis: a newly established parasitic disease in Sri Lanka. (2008). Journal of the Ruhunu Clinical Society, 15:3-5.
  • Siriwardana HVYD and Karunaweera ND. Leishmaniasis in Sri Lanka. (2005). Asian Parasitology. 4:257-261.
  • Kothalawala HS, Karunaweera ND. (2016). Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for Leishmania donovani infections in Sri Lanka. Ceylon Medical Journal 61: 68-70. http://doi.org/10.4038/cmj.v61i2.8286
  • Samaranayake N, Fernando SD, Neththikumara NF, Rodrigo C, Karunaweera ND, Dissanayake VHW. (2016). Association of HLA class I and II genes with cutaneous leishmaniasis: a case-control study from Sri Lanka and a systematic review. BMC Infectious Diseases 16:292. doi:10.1186/s12879-016-1626-8.
  • Refai FW, Madarasingha NP, Fernandopulle R, Karunaweera ND. (2016). Non-responsiveness to standard treatment in cutaneous leishmaniasis: A case series from Sri Lanka. Tropical Parasitology 6:155-158. http://doi.org/10.4103/2229-5070.190835
  • Kariyawasam KKGDUL, Siriwardana HVYD, Edirisuriya CS, Senerath U, Karunaweera ND. (2015). Characterization of cutaneous leishmaniasis in Matara district, Southern Sri Lanka: Evidence for case clustering. Pathogens and Global Health. http://dx.doi.org.ezp-prod1.hul.harvard.edu/10.1179/2047773215Y.0000000032
  • Senanayake SASC, Abeyewickrema W, Dotson E, Karunaweera ND. (2015). Characteristics of phlebotomine sandflies in selected areas in Sri Lanka. Southeast Asian Journal of Tropical Medicine and Public Health. 46(6): 994-1004.
  • Siriwardana HVYD, Senerath U, Chandrawansa PH, Karunaweera ND. (2015). Use of a clinical scoring system for enhanced case detection of cutaneous leishmaniasis in Sri Lanka. Pathogens and Global Health, 109 (4):174-183.
  • Siriwardana HVYD, Thalagala N, Karuanweera ND. (2010). Clinical and epidemiological studies on the cutaneous leishmaniasis caused by Leishmania (Leishmania) donovani in Sri Lanka. Annals of Tropical Medicine & Parasitology, 104(3):213–223.
  • Rathnayake D, Ranawake RR, Sirimanna G, Siriwardhane Y, Karunaweera ND, De Silva R. (2010). Co-infection of mucosal leishmaniasis and extra pulmonary tuberculosis in a patient with inherent immune deficiency. International Journal of Dermatology, 49(5):549-551.
  • Ihalamulla RL, Rajapaksa US, Siriwardena HVYD, Chance M, Karunaweera ND. (2009). A simple, cost-effective method for isolation and transportation of Leishmania parasites. Ceylon Medical Journal. 54(2):46-47.
  • Ihalamulla RL, Siriwardana HVYD, Karunaweera ND. (2008). Efficacies of RPMI 1640 and M 199 media in the isolation of Leishmania from cutaneous lesions. Annals of Tropical Medicine and Parasitology, 102(2):173-175.
  • Siriwardana HVYD, Noyes H, Beeching N, Chance ML, Karunaweera ND, Bates PA. (2007). L.donovani and cutaneous leishmaniasis, Sri Lanka. Emerging Infectious Diseases, 13(3):476-478.
  • Rajapaksa US, Ihalamulla RL, Udagedera C, Karunaweera ND. (2007). Cutaneous leishmaniasis in southern Sri Lanka. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101(8):799-803.
  • Ihalamulla RL, Rajapaksa U, Karunaweera ND. (2006). Micro culture for the isolation of Leishmania, modified to increase efficacy: a follow-up to a previous study. Annals of Tropical Medicine and Parasitology, 100(1):87-9.
  • Ihalamulla RL, Rajapaksa U, Karunaweera ND. (2005). Micro culture for the isolation of Leishmania parasites from cutaneous lesions — Sri Lankan experience. Annals of Tropical Medicine and Parasitology, 99(6):571-5.
  • Rajapksa US, Ihalamulla RL, Karunaweera ND. (2005). First report of mucosal tissue localisation of leishmaniasis in Sri Lanka. Ceylon Medical Journal, 50(2):90-1.
  • Karunaweera ND, Liyanage CPG, Ihalamulla RL. (2004). A simple device to maintain in vitro cultures of Leishmania in tropical countries. Transactions of the Royal Society of Tropical Medicine and Hygiene 98(5):315-317.
  • Karunaweera ND, Pratlong F, Siriwardane HVYD, Ihalamulla RL, Dedet JP (2003). Sri Lankan cutaneous leishmaniasis is caused by Leishmania donovani zymodeme MON-37. Transactions of the Royal Society of Tropical Medicine and Hygiene, 97(4):380-381.
  • Siriwardana HVYD, Udagedara C, Karunaweera ND. Clinical features, risk factors and efficacy of cryotherapy in cutaneous leishmaniasis in Sri Lanka. (2003). The Ceylon Medical Journal, 48(1):10-12.
  • Ihalamulla RL, Siriwardane HVYD, Gamage S, Perera AJ, Karunaweera ND (2002). First successful in vitro culture of autochthonous leishmaniasis cases in Sri Lanka. The Ceylon Medical Journal, 47(2):58.

PROMOTING AWARENESS

Promoting awareness on ‘local transmission of leishmaniasis in Sri Lanka’ among local policymakers, scientists, professionals and the international community and development of a ‘national framework for its prevention and control

  1. 2009 February (Colombo): International Colloquium on Leishmaniasis in Sri Lanka, organized by the University of Colombo (Sponsored by National Science Foundation, TEHDA. Featuring invited experts from India, Brazil and France. Attended by stakeholders from all 9 provinces. Included lectures, poster presentations of research, and development of a national action plan for prevention and control of cutaneous leishmaniasis.
  2. 2013 March (Colombo): 2nd International Colloquium on Leishmaniasis in Sri Lanka, organized by the University of Colombo. Sponsored by NSF. Features invited experts from India. Attended by stakeholders from all relevant sectors. Updated the national action plan and also included visceral leishmaniasis.

FUNDING SUPPORT

  • University of Colombo AP/3/2011/CG/01
  • National Science Foundation RG/SIDA/2002/M/01; RG/SIDA/2003/M/01; RG/2005/HS/07; RG/2011/HS/13
  • National Institute of Health USA R01AI099602: Significant as the 1st NIH grant awarded to a Sri Lankan University