Dr. Roshan Niloofa
Department of Zoology and Environment Sciences
Publications
Seroconversion following vaccination with tetanus toxoid, to diagnose specific antibody deficiency in patients with recurrent infections
Niloofa MJR, Munasinghe TMJ, Senanayake MP, De Silva NR. Seroconversion
following vaccination with tetanus toxoid, to diagnose specific antibody deficiency
in patients with recurrent infections. Proceedings of the 5th Annual scientific
sessions of Institute of Biochemistry, Molecular Biology and Biotechnology,
University of Colombo, Abstract no.16, pp 33
Determination of serum lipid peroxide and NOx level in severe leptospirosis patients in Sri Lanka
Walttan T, Fernando GTG, Niloofa MJR, Wickramasinghe H, Dikmadugoda N,
Karunanayake L, De Silva HJ, Premawansa S, Rajapakse S, Handunnetti SM.
Determination of serum lipid peroxide and NOx level in severe leptospirosis patients
in Sri Lanka. Proceedings of the 6th Annual scientific sessions of Institute of
Biochemistry, Molecular Biology and Biotechnology, University of Colombo,
Abstract no.15, pp 23
A diagnostic scoring model for leptospirosis in resource limited settings - A prospective study
Senaka Rajapakse, Praveen Weeratunga, M.J. Roshan Niloofa, Narmada Fernando,
Chathuraka Rodrigo, Sachith Maduranga, Nipun Lakshitha de Silva, H. Janaka De
Silva, Lilani Karunanayake, Shiroma Handunnetti. A diagnostic scoring model for
leptospirosis in resource limited settings – A prospective study. PLOS Neglected
Tropical Diseases.
Real-time qPCR as a reference standard test for diagnosis of leptospirosis
Niloofa R, Karunanayake L, de Silva HJ, Premawansa S, Rajapakse S, Handuunnetti
S. Real-time qPCR as a reference standard test for diagnosis of leptospirosis. 10 th
International Leptospirosis Society Conference 2017. 27 th November – 1 st December
2017; ID 84819
Serovar diversity of pathogenic Leptospira infecting patients in Western Province, Sri Lanka
Roshan Niloofa, Lilani Karunanayake, Janaka H de Silva, Senaka Rajapakse, Sunil
Premawansa, Shiroma Handunnetti.Serovar diversity of pathogenic Leptospira
infecting patients in Western Province, Sri Lanka. Proceedings of 8 th Biannual
Scientific Session of the Allergy and Immunology Society of Sri Lanka. 14 th – 15 th July,
2016;25.
Diagnostic accuracies of six different immunoassays compared to the gold standard tests, real-time quantitative PCR and isolation of pathogenic Leptospira
Roshan Niloofa, Lilani Karunanayake, Janaka H de Silva, Senaka Rajapakse, Sunil
Premawansa, Shiroma Handunnetti. Diagnostic accuracies of six different
immunoassays compared to the gold standard tests, real-time quantitative PCR and
isolation of pathogenic Leptospira. 8 th Annual Scientific Sessions of Institute of
Biochemistry, Molecular Biology and Biotechnology. 27 th May 2016;OP 08:19.
Biomarkers of oxidative stress in severe leptospirosis patients in Sri Lanka.
Narmada Fernando, Shalini Wickramasinghe, Roshan Niloofa, Chathuraka Rodrigo,
Lilani Karunanayake, H Janaka de Silva, Rajitha Wickramasinghe, Sunil
Premawansa, Senaka Rajapakse, Shiroma Handunnetti. Biomarkers of oxidative
stress in severe leptospirosis patients in Sri Lanka. PLOS ONE. 2016 June 11
Vascular endothelial cell damage by pathogenic Leptospira
Narmada Fernando, Jayamini Harasgama, Roshan Niloofa, Lilani Karunanayake,
Janaka H de Silva, Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti.
Vascular endothelial cell damage by pathogenic Leptospira.. Peradeniya University
international research sessions – iPURSE. 5 th -6 th November 2015
Superoxide production by differentiated HL-60 cells interacted with Leptospira. 2 nd ELS meeting on Leptospirosis and other rodent borne haemorrhagic fevers.
Narmada Fernando, Roshan Niloofa, Lilani Karunanayake, H Janaka de Silva,
Sunil Premawansa, Senaka Rajapakse, Shiroma Handunnetti. Superoxide production
by differentiated HL-60 cells interacted with Leptospira. 2 nd ELS meeting on
Leptospirosis and other rodent borne haemorrhagic fevers.16-18 th April 2015; 58
Role of antibody response in disease severity of leptospirosis
Roshan Niloofa, Narmada Fernando, Lilani Karunanayake, H Janaka de Silva, Sunil
Premawansa, Senala Rajapakse, Shiroma Handunnetti. Role of antibody response in
disease severity of leptospirosis. 7 th Annual Scientific Sessions of Institute of
Biochemistry, Molecular Biology and Biotechnology. 21 st May 2015;21.
Leptospirosis severity association with the humoral immune response. 2 nd ELS meeting on Leptospirosis and other rodent borne haemorrhagic fevers
Roshan Niloofa, Narmada Fernando, Lilani Karunanayake, H Janaka de Silva,
Sunil Premawansa, Senaka Rajapakse, Shiroma Handunnetti. Leptospirosis severity
association with the humoral immune response. 2 nd ELS meeting on Leptospirosis and
other rodent borne haemorrhagic fevers.16-18 th April 2015;56
Laboratory diagnosis of leptospirosis: Choice of tests at different stages of the infection
Roshan Niloofa, Narmada Fernando, Lilalni Karunanayake, H Janaka de Silva,
Sunil Premawansa, Senaka Rajapakse, Shiroma Handunnetti. Laboratory diagnosis
of leptospirosis: Choice of tests at different stages of the infection. Proceedings of 48 th Annual Academic sessions of Ceylon College of Physicians. 18 th -19 th September 2015. Abstract No 08. pp 101
Effect of Leptospira pathogenesity/ virulence on superoxide production in HL 60-derived neutrophils.
Narmada Fernando, Roshan Niloofa, Lilani Karunanayake, Janaka H De Silva,
Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti. Effect of Leptospira
pathogenesity/ virulence on superoxide production in HL 60-derived neutrophils.7 Accepted. 71 st Annual Scientific Sessions of Sri Lanka Association for the
Advancement of Science (SLAAS). 1 st -4 th December 2015.
Do antibodies play a role in ROS production by HL 60-derived neutrophils against Leptospira?
Narmada Fernando, Roshan Niloofa, Lilani Karunanayake, H Janaka de Silva, Sunil
Premawansa, Senaka Rajapakse, Shiroma Handunnetti, Do antibodies play a role in
ROS production by HL 60-derived neutrophils against Leptospira?, Proceedings of
the 7th Annual Scientific Sessions of the IBMBB, University of Colombo, 2015,
Abstract no.08, pp 18
Differentiation of severe leptospirosis from critical phase dengue infections.
Narmada Fernando, Roshan Niloofa, Lilani Karunanayake, H Janaka de Silva,
Sunil Premawansa, Senaka Rajapakse, Shiroma Handunnetti. Differentiation of
severe leptospirosis from critical phase dengue infections. 2 nd ELS meeting on
Leptospirosis and other rodent borne haemorrhagic fevers.16-18 th April 2015; 14
Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test
Roshan Niloofa, Narmada Fernando, Nipun Lakshitha de Silva, Lilani
Karunanayake, Hasith Wickremesinghe, Nandana Dikmadugoda, Gayani
Premawansa, Rajitha Wikramasinghe, H Janaka de Silva, Sunil Premawansa, Senaka
Rajapakse, Shiroma Handunnetti. Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test. PLOS ONE. 2015 Jun 18;10 (6):e0129236
Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test
Roshan Niloofa, Narmada Fernando, Nipun Lakshitha de Silva, Lilani
Karunanayake, Hasith Wickremesinghe, Nandana Dikmadugoda, Gayani
Premawansa, Rajitha Wikramasinghe, H Janaka de Silva, Sunil Premawansa, Senaka
Rajapakse, Shiroma Handunnetti. Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test. PLOS ONE. 2015 Jun 18;10 (6):e0129236
Clinico-epidemiological characteristics, treatment and outcomes of patients with confirmed Leptospirosis in a Sri Lankan healthcare setting
Rajapakse S, Weeratunga PN, Rodrigo C, Sriharan S, Niloofa MJR, Fernando N, de
Silva HJ, Karunanayake L, Premawansa S, Handuunnetti S. Clinico-epidemiological
characteristics, treatment and outcomes of patients with confirmed Leptospirosis in a
Sri Lankan healthcare setting. 128 th Anniversary International Medical Congress of
Sri Lankan Medical Association. 6 th -8 th July 2015; OP 009,120.
Clinical and laboratory associations of severity in a Sri Lankan cohort of patients with serologically confirmed Leptospirosis- a prospective study.
Rajapakse S, Weeratunga PN, Rodrigo C, Sriharan S, Niloofa MJR, Fernando N, de
Silva HJ, Karunanayake L, Premawansa S, Handuunnetti S. Clinical and laboratory
associations of severity in a Sri Lankan cohort of patients with serologically
confirmed Leptospirosis- a prospective study. 128 th Anniversary International
Medical Congress of Sri Lankan Medical Association. 6 th -8 th July 2015; OP 007,119.
Clinical and laboratory associations of severity in a Sri Lankan cohort of patients with serologically confirmed leptospirosis – A prospective study
Senaka Rajapakse, Praveen Weeratunga, M.J. Roshan Niloofa, Narmada Fernando,
Chathuraka Rodrigo, Sachith Maduranga, Nipun Lakshitha Fernando, H. Janaka De
Silva, Lilani Karunanayake, Shiroma Handunnetti . Clinical and laboratory
associations of severity in a Sri Lankan cohort of patients with serologically
confirmed leptospirosis – A prospective study. Trans R Soc Trop Med Hyg. 2015
Nov;109(11):710-6. doi: 10.1093/trstmh/trv079.
A diagnostic model for Leptospirosis for use in resource limited settings.
Rajapakse S, Weeratunga PN, Rodrigo C, Sriharan S, Niloofa MJR, Fernando N, de
Silva HJ, Karunanayake L, Premawansa S, Handuunnetti S. A diagnostic model for
Leptospirosis for use in resource limited settings. 128 th Anniversary International
Medical Congress of Sri Lankan Medical Association. 6 th -8 th July 2015; OP 008,120.
Oxidative damage of lipids and proteins in leptospirosis. 2 nd International Conference on Frontiers in Molecular Life Sciences, IBMBB, University of Colombo
Fernando TRGN, Niloofa MJR, Maduranga S, Rodrigo C, Karunanayake L, De
Silva HJ, Rajapakse S, Premawansa S, Handunnetti SM. Oxidative damage of lipids
and proteins in leptospirosis. 2 nd International Conference on Frontiers in Molecular
Life Sciences, IBMBB, University of Colombo, 10-11 th September 2014;38
Neutrophil leukocytosis and low haemoglobin may predict severe disease in leptospirosis
Nipun Lakshitha De Silva, MJR Niloofa, Narmada Fernando, Lilani Karunanayake,
Chaturaka Rodrigo, H Janaka de Silva, Sunil Premawansa, Shiroma M Handunnetti,
Senaka Rajapaksa. Neutrophil leukocytosis and low haemoglobin may predict severe
disease in leptospirosis, International Archives of Medicine. 2014:7:31
High serum lipid peroxide and low anti-oxidant capacity in leptospirosis
Narmada Fernando, Niloofa MJR, Sachith Maduranga, Lilani Karunanayake, Janaka
De Silva, Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti, High serum
lipid peroxide and low anti-oxidant capacity in leptospirosis, One Health
International Conference, University of Peradeniya; 2014; pp 20
Evaluation of two rapid immunodiagnostic tests, an IgM ELISA and an immunochromatography test compared to microscopic agglutination test.
Niloofa MJR, Fernando TRGN, De Silva NL, Karunanayake L, Wickremesinghe
H, Dikmadugoda N, Premawansa G, Wikramasinghe AR, De Silva HJ, Premawansa
S, Rajapakse S, Handunnetti SM. Evaluation of two rapid immunodiagnostic tests, an
IgM ELISA and an immunochromatography test compared to microscopic
agglutination test. 2 nd International Conference on Frontiers in Molecular Life
Sciences, IBMBB, University of Colombo, 10-11 th September 2014;38
Evaluation of Leptospirosis diagnostic tests: microscopic agglutination test, IgM based ELISA and immunochromatography test
Niloofa MJR, Fernando TRGN, De Silva NL, Karunanayake L, De Silva HJ,
Premawansa S, Rajapakse S, Handunnetti SM . Evaluation of Leptospirosis diagnostic
tests: microscopic agglutination test, IgM based ELISA and immunochromatography
test. Immunology Day Symposium 2014, Allergy and Immunology Society of Sri
Lanka, 26 th February, 2014.
Evaluation of IgM ELISA for diagnosis of acute leptospirosis using pathogenic Leptospira antigens
Niloofa MJR, Karunanayake L, Wikramasinghe AR, De Silva HJ, Premawansa S,
Rajapakse S, Handunnetti SM. Evaluation of IgM ELISA for diagnosis of acute
leptospirosis using pathogenic Leptospira antigens. 34 th Annual Session of the
Institute of Biology, Sri Lanka. 26 th September 2014;58.
Establishment and validation of an in-house IgM ELISA for diagnosis of acute leptospirosis
Niloofa MJR, Karunanayake L, Wikramasinghe AR, De Silva HJ, Premawansa S,
Rajapakse S, Handunnetti SM. Establishment and validation of an in-house IgM
ELISA for diagnosis of acute leptospirosis. Proceedings of 7 th Biannual Scientific
Session of the Allergy and Immunology Society of Sri Lanka. 2 nd September 2014;27
Rapid detection of severe leptospirosis using a simple cost-effective method
Fernando TRGN, Handunetti SM, Niloofa MJR, Fernando GTG, De Silva NL,
Wijerathne PPB, Rodrigo C, Karunanayake L, Wickremesinghe H, Dikmadugoda N,
Premawansa G, De Silva HJ, Wickremesinghe AR, Premawansa S, Rajapakse S.
Rapid detection of severe leptospirosis using a simple cost-effective method.
Proceedings of the 46th Annual academic sessions of the Ceylon College of
Physicians, 12 th September 2013; Abstract no. OP16, pp 53.
Low serum nitrite level and anti-oxidant capacity in severe leptospirosis in Sri Lanka
Fernando TRGN, Niloofa MJR, Fernando GTG, De Silva NL, Rodrigo C,
Karunanayake L, Wickremesinghe H, Dikmadugoda N, Premawansa G, De Silva HJ,
Rajapakse S, Premawansa S, Handunnetti SM, (2013,) Low serum nitrite level and
anti-oxidant capacity in severe leptospirosis in Sri Lanka, Proceedings of the 6th
Annual scientific sessions of Institute of Biochemistry, Molecular Biology and
Biotechnology, University of Colombo, Abstract no.14, pp 22