Induction of Apoptosis in MCF‐7 Breast Cancer Cells by Sri Lankan Endemic Mango (Mangifera zeylanica) Fruit Peel through Oxidative Stress and Analysis of its Phytochemical Constituents.

Ediriweera MK, Tennekoon KH, Samarakoon SR, Thabrew I, De Silva ED. Induction of apoptosis in MCF‐7 breast cancer cells by Sri Lankan Endemic Mango (Mangifera zeylanica) fruit peel through oxidative stress and analysis of its phytochemical constituents. Journal of Food Biochemistry. 2017 Feb;41(1):e12294.
Publication year: 2017
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Abstract

In this study, in vitro cytotoxic and apoptotic effects of the organic extracts (hexane, chloroform, ethyl acetate and methanol) of Mangifera zeylanica fruit peel and flesh on estrogen receptor positive MCF-7 cancer cells were examined. Chloroform extract of the peel was found to be cytotoxic to cancer cells (IC50 78.46 ± 1.7 µg/mL) while none of the flesh extracts were cytotoxic. Caspase 7 activity was increased and DNA fragmentation as well as morphological features of apoptosis shown by acridine orange/ethidium bromide and Hoechst (33258) staining occurred in response to the chloroform extract of the peel. Peel chloroform extract also increased production of reactive oxygen species, malondialdehyde, glutathione-S-transferase and super oxide dismutase and depleted glutathione levels. The overall results of our study suggest that the peel chloroform extract can induce breast cancer cell apoptosis, perhaps by activation of an oxidative stress mechanism.

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