n vitro study of IgE independent basophil degranulation due to selected food items

Oral Presentations
Wijerathna DMCK, Handunnetti SM, Fernando N, Premawansa S
Publication year: 2019

Wijerathna DMCK, Handunnetti SM, Fernando N, Premawansa S, In vitro study of IgE independent basophil degranulation due to selected food items, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 21

Ion pump and ion channel modulators as anti-mycobacterial agents

Poster Presentation
Mitini-Nkhoma SC, Pathirana PPSL, Fernando N, Ishaka D, Handunnetti SM
Publication year: 2019

Mitini-Nkhoma SC, Pathirana PPSL, Fernando N, Ishaka D, Handunnetti SM, Ion pump and ion channel modulators as anti-mycobacterial agents, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 34

In vitro effects of methanol-dichloromethane extract of Vernonia zeylanica and its solvent fractions on human, matured KU 812-derived basophils

Oral Presentations
Ezechukwu HC, Rukshala BAD, Dilini I, Fernando N, Handunnetti SM
Publication year: 2019

Ezechukwu HC, Rukshala BAD, Dilini I, Fernando N, Handunnetti SM, In vitro effects of methanol-dichloromethane extract of Vernonia zeylanica and its solvent fractions on human, matured KU 812-derived basophils, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 26

Effects of ion pump and ion channel modulators on activation and function THP-1 derived macrophages

Oral Presentations
Mitini-Nkhoma SC, Pathirana PPSL, Fernando N, Handunnetti SM, Ishaka D
Publication year: 2019

Mitini-Nkhoma SC, Pathirana PPSL, Fernando N, Handunnetti SM, Ishaka D, Effects of ion pump and ion channel modulators on activation and function THP-1 derived macrophages, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 25

Comparison of IgG and IgM responses in rats immunized with two types of antigen preparations of Leptospira biflexa serovar Patoc

Oral Presentations
Gangani PD, Ishaka GKD, Fernando N, Karunanayake L, Rajapakse S, Premawansa S, Handunnetti SM
Publication year: 2019

Gangani PD, Ishaka GKD, Fernando N, Karunanayake L, Rajapakse S, Premawansa S, Handunnetti SM, Comparison of IgG and IgM responses in rats immunized with two types of antigen preparations of Leptospira biflexa serovar Patoc, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 22

 

A preliminary study on VP1 gene based genotyping of BK virus isolated from kidney transplant patients

Oral Presentations
Ratnayake AKDVY, Jayamaha CJS, Fernando N, Gajanayake T
Publication year: 2019

Ratnayake AKDVY, Jayamaha CJS, Fernando N, Gajanayake T, A preliminary study on VP1 gene based genotyping of BK virus isolated from kidney transplant patients, International Conference on Infectious Diseases and Antimicrobial Resistance, Sri Lanka Collage of Microbiologists, Aug 08th – 11th; pp 14

 

A preliminary study on the roles of oxidative stress and anti-oxidant therapy in resistant hypertension patients in Sri Lanka

Poster Presentation
Ranasinghe RAHN, Fernando N, Handunnetti S, Weeratunga P, Galappatthy P, Constantine GR
Publication year: 2019

Ranasinghe RAHN, Fernando N, Handunnetti S, Weeratunga P, Galappatthy P, Constantine GR, A preliminary study on the roles of oxidative stress and anti-oxidant therapy in resistant hypertension patients in Sri Lanka, Proceedings of the 11th Annual Scientific Sessions, IBMBB, University of Colombo, 2019, pp 35

 

The effect of antimicrobial agents on inflammatory cytokines in acute leptospirosis

Publications in peer reviewed Journals
Narmada Fernando, Rajiva de Silva, Shiroma M Handunnetti, Lilani Karunanayake, Nipun Lakshitha De Silva, H Janaka de Silva, Senaka Rajapakse, Sunil Premawansa
Publication year: 2018

Narmada Fernando, Rajiva de Silva, Shiroma M Handunnetti, Lilani Karunanayake, Nipun Lakshitha De Silva, H Janaka de Silva, Senaka Rajapakse, Sunil Premawansa. The effect of antimicrobial agents on inflammatory cytokines in acute leptospirosis. Antimicrobial Agents and Chemotherapy. May 2018; 62(5) e02312-17. r62:e02312-17.https://doi .org/10.1128/ AAC.02312-17.

Serological prevalence and determination of cutoff levels of Leptospirosis IgM and IgG in relation to Patoc I strain of Leptospira biflexa in a cohort of individuals from a high prevalence region in Sri Lanka

Poster Presentation
Balaji K, Weeratunga  PN,  Ramchandani KC, Wijerathne PBP, Sam G, Fernando N, Handunnetti SM, Fernando SD,  Rajapakse S
Publication year: 2018

Balaji K, Weeratunga  PN,  Ramchandani KC, Wijerathne PBP, Sam G, Fernando N,
Handunnetti SM, Fernando SD,  Rajapakse S, Serological prevalence and determination of cutoff levels of Leptospirosis IgM and IgG in relation to Patoc I strain of Leptospira biflexa in a cohort of individuals from a high prevalence region in Sri Lanka, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp

Microscopic Agglutination Titre (MAT) for leptospirosis and associations in a population cohort in a high endemic region in Sri Lanka

Poster Presentation
Balaji K, Weeratunga  PN,  Ramchandani KC, Wijerathne PBP, Sam G, Fernando N, Handunnetti SM, Fernando SD,  Rajapakse S
Publication year: 2018

Balaji K, Weeratunga  PN,  Ramchandani KC, Wijerathne PBP, Sam G, Fernando N,
Handunnetti SM, Fernando SD,  Rajapakse S, Microscopic Agglutination Titre (MAT) for leptospirosis and associations in a population cohort in a high endemic region in Sri Lanka, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp

In vitro study on the effect of antimicrobial agents on inflammatory cytokine response in leptospirosis

Oral Presentations
Sam G, Fernando N, Rajapakse S, Handunnetti SM
Publication year: 2018

Sam G, Fernando N, Rajapakse S, Handunnetti SM, In vitro study on the effect of antimicrobial agents on inflammatory cytokine response in leptospirosis, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp 25

Direct degranulation of rat mast cells (RBL-2H3) and increase in generation of superoxide anion (O2-) induced by aqueous extract of Acacia pollen and Apis dorsata venom

Oral Presentations
Ishaka GKD, Gangani PD, Rukshala BAD, Gunasekara DLPE, Fernando N, Handunnetti SM
Publication year: 2018

Ishaka GKD, Gangani PD, Rukshala BAD, Gunasekara DLPE, Fernando N, Handunnetti SM, Direct degranulation of rat mast cells (RBL-2H3) and increase in generation of superoxide anion (O2-) induced by aqueous extract of Acacia pollen and Apis dorsata venom, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp 20

Development of a Colourimetric Reverse Transcription Loop-mediated Isothermal Amplification assay for the rapid detection of different Serotypes of the Dengue virus

Oral Presentations
Ariyaratne MHJD, Nguyen A, De Silva AD, Fernando N, Weerasena OVDSJ, Handunnetti SM
Publication year: 2018

Ariyaratne MHJD, Nguyen A, De Silva AD, Fernando N, Weerasena OVDSJ, Handunnetti SM, Development of a Colourimetric Reverse Transcription Loop-mediated Isothermal Amplification assay for the rapid detection of different Serotypes of the Dengue virus, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp 26

Comparison of a two-step and a novel single step method for neutrophil isolation

Poster Presentation
Lakmali AKD , Fernando N, Handunnetti SM, Premawansa S
Publication year: 2018

Lakmali AKD , Fernando N, Handunnetti SM, Premawansa S, Comparison of a two-step and a novel single step method for neutrophil isolation, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018

A preliminary study on VP1 gene based genotyping of BK virus isolated from kidney transplant patients

Poster Presentation
Ratnayake AKDVY, Jayamaha CJS, Fernando N, Gajanayake T
Publication year: 2018

Ratnayake AKDVY, Jayamaha CJS, Fernando N, Gajanayake T, A preliminary study on VP1 gene based genotyping of BK virus isolated from kidney transplant patients, Proceedings of the 10th Annual Scientific Sessions, IBMBB, University of Colombo, 2018, pp

 

The in vitro effect of pathogenic Leptospiraon FOXP3 expression in lymphocytes

Oral Presentations
Dayarathna HMSP, Fernando N, Handunnetti SM, Weerasena OVDSJ
Publication year: 2017

Dayarathna HMSP, Fernando N, Handunnetti SM, Weerasena OVDSJ. The in vitro effect of pathogenic Leptospiraon FOXP3 expression in lymphocytes. Proceedings of the 9th Annual Scientific Sessions, IBMBB, University of Colombo, 2017, pp 17.

The effect of Munronia pinnata on Natural Killer (NK-92MI) cell

Poster Presentation
Fernando AN, Fernando N, Handunnetti SM, Hapuaarachchi S
Publication year: 2017

Fernando AN, Fernando N, Handunnetti SM, Hapuaarachchi S, The effect of Munronia pinnata on Natural Killer (NK-92MI) cells, Proceedings of the 9th Annual Scientific Sessions, IBMBB, University of Colombo, 2017, pp 33.

 

Multilocus Sequence Typing of Leptospira from clinical isolates in Sri Lanka

Poster Presentation
Karunanayake Lilani, Vimal Raj R, Anwesh M, Muruganandam N, Perera R, Niloofa R, Fernando N, Handunnetti S. Premawansa S, Rajapakse S., Vijayachari P
Publication year: 2017

Karunanayake Lilani, Vimal Raj R, Anwesh M, Muruganandam N, Perera R, Niloofa R, Fernando N, Handunnetti S. Premawansa S, Rajapakse S., Vijayachari P.  Multilocus Sequence Typing of Leptospira from clinical isolates in Sri Lanka, 10th International Leptospirosis Society Scientific Meeting, Palmerstone, New Zealand, 27th Nov-1stDec 2017, pp 251

 

Leptospirosis: challenges in diagnosis, and predictors of severity

Publications in peer reviewed Journals
Senaka Rajapakse, Narmada Fernando, MJR Niloofa, H. Janaka de Silva, Lilani Karunanayake, Sunil Premawansa, Shiroma Handunnetti
Publication year: 2017

Senaka Rajapakse, Narmada Fernando, MJR Niloofa, H. Janaka de Silva, Lilani Karunanayake, Sunil Premawansa, Shiroma Handunnetti (2017) Leptospirosis: challenges in diagnosis, and predictors of severity. Journal of the Ceylon College of Physicians. 47(2), pp.60–69.

Establishment of methods for retrieving Leptospira organisms/ antigens in urine and blood

Poster Presentation
Weerasinghe HS, Fernando N, Rajapakse S, Premawansa S, Handunnetti SM
Publication year: 2017

Weerasinghe HS, Fernando N, Rajapakse S, Premawansa S, Handunnetti SM, Establishment of methods for retrieving Leptospira organisms/ antigens in urine and blood, Proceedings of the 9th Annual Scientific Sessions, IBMBB, University of Colombo, 2017, pp 31.

 

Do human serum factors protect endothelial cells against pathogenic Leptospira?

Poster Presentation
Narmada Fernando, Shiroma Handunnetti, Lilani Karunanayake, Sunil Premawansa, Senaka Rajapakse.
Publication year: 2017

Narmada Fernando, Shiroma Handunnetti, Lilani Karunanayake, Sunil Premawansa, Senaka Rajapakse. Do human serum factors protect endothelial cells against pathogenic Leptospira?, 10th International Leptospirosis Society Scientific Meeting, Palmerstone, New Zealand, 27th Nov-1stDec 2017, p 245

Assessment of RNS and ROS production by healthy phagocytic cells stimulated with leptospires in vitro

Poster Presentation
Wickramasinghe Arachchi SM, Edward D, Fernando N, Hadunnetti SM, Rajapakse S
Publication year: 2017

Wickramasinghe Arachchi SM, Edward D, Fernando N, Hadunnetti SM, Rajapakse S. Assessment of RNS and ROS production by healthy phagocytic cells stimulated with leptospires in vitro. Proceedings of the 9th Annual Scientific Sessions, IBMBB, University of Colombo, 2017, pp 30.

Antibiotic Susceptibility of Clinical Isolates of Human Leptospirosis in Sri Lanka

Poster Presentation
Karunanayake Lilani, Rajapaksha RRN, Perera KCR, Fernando N, Niloofa R, Handunnetti S, Premawansa S, Rajapakse S
Publication year: 2017

Karunanayake Lilani, Rajapaksha RRN, Perera KCR, Fernando N, Niloofa R, Handunnetti S, Premawansa S, Rajapakse S. Antibiotic Susceptibility of Clinical Isolates of Human Leptospirosis in Sri Lanka, 10th International Leptospirosis Society Scientific Meeting, Palmerstone, New Zealand, 27th Nov-1stDec 2017, pp 203

Altered Reactive Nitrogen Species production, NF-κB expression in phagocytes and severity of severe leptospirosis infections

Poster Presentation
Shiroma Handunnetti, Narmada Fernando, Sanjika Milhani, Daniya Edward, Lilani Karunanayake, Sunil Premawansa, Senaka Rajapakse
Publication year: 2017

Shiroma Handunnetti, Narmada Fernando, Sanjika Milhani, Daniya Edward, Lilani Karunanayake, Sunil Premawansa, Senaka Rajapakse. Altered Reactive Nitrogen Species production, NF-κB expression in phagocytes and severity of severe leptospirosis infections, 10th International Leptospirosis Society Scientific Meeting, Palmerstone, New Zealand, 27th Nov-1stDec 2017, pp 247

Protein Carbonyl as a Biomarker of Oxidative Stress in Severe Leptospirosis, and Its Usefulness in Differentiating Leptospirosis from Dengue Infections

Publications in peer reviewed Journals
Narmada Fernando, Shalini Wickremesinghe, Roshan Niloofa, Chaturaka Rodrigo, Lilani Karunanayake, H. Janaka de Silva, A. R. Wickremesinghe, Sunil Premawansa, Senaka Rajapakse, Shiroma M. Handunnetti
Publication year: 2016

Narmada Fernando, Shalini Wickremesinghe, Roshan Niloofa, Chaturaka Rodrigo, Lilani Karunanayake, H. Janaka de Silva, A. R. Wickremesinghe, Sunil Premawansa, Senaka Rajapakse, Shiroma M. Handunnetti (2016) Protein Carbonyl as a Biomarker of Oxidative Stress in Severe Leptospirosis, and Its Usefulness in Differentiating Leptospirosis from Dengue Infections. PLoS ONE 11(6): e0156085. doi:10.1371/journal. pone.0156085

Protein Carbonyl as a Biomarker of Oxidative Stress for Differentiating Critical Phase Dengue Infections from Severe Leptospirosis

Poster Presentation
Narmada Fernando, Shalini Wickremesinghe, Roshan Niloofa, Chaturaka Rodrigo, Lilani Karunanayake, H. Janaka de Silva, A. R. Wickremesinghe, Sunil Premawansa, Senaka Rajapakse, Shiroma M. Handunnetti
Publication year: 2016

Narmada Fernando, Shalini Wickremesinghe, Roshan Niloofa, Chaturaka Rodrigo, Lilani Karunanayake, H. Janaka de Silva, A. R. Wickremesinghe, Sunil Premawansa, Senaka Rajapakse, Shiroma M. Handunnetti, Protein Carbonyl as a Biomarker of Oxidative Stress for Differentiating Critical Phase Dengue Infections from Severe Leptospirosis, NSF Research Summit, 2016, Abstract no. DE 01.

Effects of pathogenic Leptospira on functions and viability of vascular endothelial cells and kidney epithelial cells

Oral Presentations
Fernando N., Handunnetti SM., Rajapakse S., de  Silva HJ and Premawansa S
Publication year: 2016

Fernando N., Handunnetti SM., Rajapakse S., de  Silva HJ and Premawansa S. Effects of pathogenic Leptospira on functions and viability of vascular endothelial cells and kidney epithelial cells. Proceedings of the 8th Annual Scientific Sessions, IBMBB, University of Colombo, 2016, pp 20.

A diagnostic scoring model for leptospirosis in resource limited settings - A prospective study

Publications in peer reviewed Journals
Senaka Rajapakse, Praveen Weeratunga, Roshan Niloofa, Narmada Fernando, Nipun Lakshitha de Silva, Chaturaka Rodrigo, Sachith Maduranga, Nuwanthi Nandasiri, Sunil Premawansa, Lilani Karunanayake, H. Janaka de Silva, Shiroma Handunnetti
Publication year: 2016

Senaka Rajapakse, Praveen Weeratunga, Roshan Niloofa, Narmada Fernando, Nipun Lakshitha de Silva, Chaturaka Rodrigo, Sachith Maduranga, Nuwanthi Nandasiri, Sunil Premawansa, Lilani Karunanayake, H. Janaka de Silva, Shiroma Handunnetti (2016) A diagnostic scoring model for leptospirosis in resource limited settings – A prospective study. PLOS Neglected Tropical Diseases. 10 (6): e0004513. doi:10.1371/journal.pntd.0004513

Vascular endothelial cell damage by pathogenic Leptospira

Oral Presentations
Narmada Fernando, Jayamini Harasgama, Roshan Niloofa, Lilani Karunanayake, Janaka H de Silva, Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti
Publication year: 2015

Narmada Fernando, Jayamini Harasgama, Roshan Niloofa, Lilani Karunanayake, Janaka H de Silva, Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti. Vascular endothelial cell damage by pathogenic Leptospira. iPURSE 2015, University of Peradeniya; pp 339

 

The role of neutrophils in the interaction between pathogenic Leptospira and vascular endothelial cells

Poster Presentation
Shiroma Handunnetti, Narmada Fernando, Senaka Rajapakse, H Janaka de Silva, Sunil Premawansa
Publication year: 2015

Shiroma Handunnetti, Narmada Fernando, Senaka Rajapakse, H Janaka de Silva, Sunil Premawansa, The role of neutrophils in the interaction between pathogenic Leptospira and vascular endothelial cells, 9th International Leptospirosis Society Scientific Meeting 2015, Semarang, Indonesia; pp 101