- Stem cells iPSCs and cancer stem cell culture techniques
- Cell culture techniques including media preparation, maintenance of cell lines, cryopreservation and maintenance of cell culture laboratory.
- Isolation and maintenance of human and rat primary cell cultures
- Cell based assays such as MTT, SRB, LDH, ApoTox-Glo™ Triplex, BrdU, and CellTox™ Green Cytotoxicity assays.
- DNA based techniques such as cloning, sequencing, genomic and cDNA library construction and screening, Polymerase Chain Reaction and reverse Transcription- PCR, real-time PCR, gel electrophoresis, RFLP and AFLP analysis.
- HPLC, TLC, GC/MS, FPLC and Heavy Metal analysis.
- Cytogenetic techniques such as chromosome aberration, micronucleus analysis and COMET assay
- Southern Blot analysis, Northern Blot analysis, Western Blot analysis, Immunohitochemistry and Fluorescent and Confocal microscopy.
- Handling of isotopes for the preparation of labeled probes.
- Ability to use Bioinformatics tools, homology modeling and molecular docking.
- Interpretation of NMR and Mass spectroscopic data and structure elucidation of new compounds