Professor Shiroma M Handunnetti
Institute of Biochemistry, Molecular Biology and Biotechnology (IBMBB)
Publications
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Diagnosis of Vespa affinis venom allergy: Use of immunochemical methods and a passive basophil activation test
Peshala Gunasekara; SM Handunnetti; Sunil Premawansa; Pradeep Kaluarachchi; Chandima Karunathilake; Indra P Ratnayake; RKS Dias; GAS Premakumara; WMDK Dasanayake; Suranjith L Seneviratne; Rajiva de Silva. Diagnosis of Vespa affinis venom allergy: Use of immunochemical methods and a passive basophil activation test. Allergy, Asthma & Clinical Immunology, 2019; 15:80 https://doi.org/10.1186/s13223-019-0394-6
Diagnosis of Vespa affinis venom allergy: use of immunochemical methods (OP10)
Gunasekara DLPE, Handunnetti SM, Premawansa S, Dasanayake WMDK, Ratnayake IP, Dias RKS, Premakumara GAS, Seneviratne SL and De Silva R. Diagnosis of Vespa affinis venom allergy: use of immunochemical methods (OP10). Proceeding of the 11th Annual Scientific Sessions of Institute of Biochemistry, Molecular Biology & Biotechnology (IBMBB), University of Colombo. 12th July 2019, pp:24
Development of a triplex reverse transcription-real time polymerase chain reaction for simultaneous detection of dengue, Japanese encephalitis and chikungunya viruses (OP14)
Perera HIT, Muzeniek T, Targosz A, Yapa W, Premawansa S, Premawansa G, Handunnetti S, Weerasena OVDSJ, Kohl C, Nitsche A. Development of a triplex reverse transcription-real time polymerase chain reaction for simultaneous detection of dengue, Japanese encephalitis and chikungunya viruses (OP14). Proceeding of the 11th Annual Scientific Sessions of Institute of Biochemistry, Molecular Biology & Biotechnology (IBMBB), University of Colombo. 12th July 2019, pp:28.
Cross-reactivity of venom of Sri Lankan ant species with Apis dorsata and Vespa affinis venom, (OP12)
Peiris TMR, Gunasekara DPLE, SM Handunnetti, Dasanayake WMDK, de Silva NR. Cross-reactivity of venom of Sri Lankan ant species with Apis dorsata and Vespa affinis venom, (OP12). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 26
Comparison of antigen profiles of pathogenic serovars of L. interrogans and non-pathogenic L. biflexa. (PP01)
WGK Anuradha, PD Gangani, L Karunanayake, N Fernando, SM Handunnetti. Comparison of antigen profiles of pathogenic serovars of L. interrogans and non-pathogenic L. biflexa. (PP01). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 27
Characterization of antigens of Enterobius vermicularis (pinworm) eggs. (OP2)
Kostha YBNS De, Pathirana SL, Handunnetti SM, Gunawardena S. Characterization of antigens of Enterobius vermicularis (pinworm) eggs. (OP2). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 16
Changes in peritoneal lymphocyte subpopulations and antibody response in rats immunized with purified LPS of pathogenic and non-pathogenic Leptospira spp. (PP04)
DDWCK Dissanayake, SM Handunnetti, N Fernando, D Gangani, RD Silva, S Premawansa. Changes in peritoneal lymphocyte subpopulations and antibody response in rats immunized with purified LPS of pathogenic and non-pathogenic Leptospira spp. (PP04). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 30
Association between serum NOx levels and antioxidant capacity levels among long-term meditators: a preliminary study. (OP5)
Thambyrajah JC, Handunnetti SM, Dilanthi WH, Dissanayake WDN. Association between serum NOx levels and antioxidant capacity levels among long-term meditators: a preliminary study. (OP5). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 19
A pilot study on allergenicity of Artocarpus heterophyllus, Moringa oleifera, and Trianthema portulacastrum based on cases reported in Sri Lanka. (OP11)
Iddagoda IDJH, Gunasekara DLPE, Handunnetti SM, Jeewandara JMC, Karunathilaka C, Malavige GN, de Silva NR, Dasanayake WMDK. A pilot study on allergenicity of Artocarpus heterophyllus, Moringa oleifera, and Trianthema portulacastrum based on cases reported in Sri Lanka. (OP11). Proceedings of the 9th Biennial Scientific Sessions, Allergy and Immunology Society of Sri Lanka, pp 25
Utility of pf/pan RDT for diagnosis in the phase of prevention of re-establishment of malaria in Sri Lanka
Kumudunayana Gunasekera, Risintha Premaratne, Jagathpriya Weerasena, Sunil Premawansa, Shiroma Handunnetti, Deepika Fernando. Utility of pf/pan RDT for diagnosis in the phase of prevention of re-establishment of malaria in Sri Lanka. Pathogens and Global Health, 2018. Oct;112(7):360-367. doi: 10.1080/20477724.2018.1536855. Epub 2018 Oct 24
Use of molecular makers for monitoring therapeutic efficacy of malaria patients in the malaria prevention of re-introduction phase in Sri Lanka
Gunasekera WMKT de AW, Premaratne RG, Weerasena OVDSJ, Handunnetti SM, Premawansa S, Fernando SD. Use of molecular makers for monitoring therapeutic efficacy of malaria patients in the malaria prevention of re-introduction phase in Sri Lanka. Proceeding of the 74th Annual Session of SLAAS, pp: 105
Use of allergenic cross-reactivity between Apis dorsata Fabricius (Giant Asian Honeybee) and Apis mellifera Linnaeus (Western Honeybee) for diagnosis of A. dorsata venom allergy. (OP07)
Gunasekara DLPE, Handunnetti SM, Premawansa S, Witharana EWRA, Dasanayake WMDK, Ratnayake IP, Seneviratne SL, Dias RKS, Premakumara GAS, De Silva R. Use of allergenic cross-reactivity between Apis dorsata Fabricius (Giant Asian Honeybee) and Apis mellifera Linnaeus (Western Honeybee) for diagnosis of A. dorsata venom allergy. (OP07). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 21
The effect of antimicrobial agents on inflammatory cytokines in acute leptospirosis
Narmada Fernando, Rajiva de Silva, Shiroma M Handunnetti, Lilani Karunanayake, Nipun Lakshitha De Silva, H Janaka de Silva, Senaka Rajapakse, Sunil Premawansa. The effect of antimicrobial agents on inflammatory cytokines in acute leptospirosis. Antimicrobial Agents and Chemotherapy. May 2018; 62(5) e02312-17. r62:e02312-17.https://doi .org/10.1128/ AAC.02312-17
Studies on in vitro anti-inflammatory potential and phytoconstituents of ethanol bark extract of Flacourtia indica. (OP14)
Perera HDSM, Samarasekera R, Handunnetti SM, Weerasena OVDSJ, Jabeen A, Choudhary MI. Studies on in vitro anti-inflammatory potential and phytoconstituents of ethanol bark extract of Flacourtia indica. (OP14). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 28
Serological prevalence and determination of cut-off levels of Leptospirosis IgM and IgG in relation to Patoc I strain of Leptospira biflexa in a cohort of individuals from a high prevalence region in Sri Lanka
Balaji K, Weeratunga PN, Ramchandani KC, Wijerathne PBP, Sam G, Fernando N, Handunnetti SM, Fernando SD, Rajapakse S. Serological prevalence and determination of cut-off levels of Leptospirosis IgM and IgG in relation to Patoc I strain of Leptospira biflexa in a cohort of individuals from a high prevalence region in Sri Lanka. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 39
Reactivity of population samples from Colombo district for anti-leptospiral antibodies in microscopic agglutination test
Balaji K, Weeratunga PN, Ramchandani KC, Wijerathne PBP, Sam G, Fernando N, Handunnetti SM, Fernando SD, Rajapakse S. Reactivity of population samples from Colombo district for anti-leptospiral antibodies in microscopic agglutination test. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 38
Preliminary study on pollen records during South-West monsoon in selected locations in Sri Lanka and direct effect of pollen on basophil degranulation
Guruge BMA, Ishaka GKD, Handunnetti SM, de Silva R, Premawansa S, Premathilake TR. Preliminary study on pollen records during South-West monsoon in selected locations in Sri Lanka and direct effect of pollen on basophil degranulation. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 34
Preliminary study on CTLA-4 gene mutations in Inflammatory Bowel Disease in Sri Lanka
Hewagama AHS, Pathirana SL, Handunnetti SM, Seneviratne S. Preliminary study on CTLA-4 gene mutations in Inflammatory Bowel Disease in Sri Lanka. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 31
Preliminary study on Chronic granulomatous disease in Sri Lanka
Fernando SJA, Faiz NM, Handunnetti SM, De Silva AD, Dasanayake WMDK, Wickremasinghe GD, Karunatilake RMCH, De Silva R. Preliminary study on Chronic granulomatous disease in Sri Lanka. Allergy Asthma & Clinical Immunology. 2018; 14: 37. doi: 10.1186/s13223-018-0264-7
Optimization of isolation of BK viral DNA from renal transplant patients and VP1 gene- based genotyping
Ratnayake AKDVY, Fernando N, Gajanayake T, Handunnetti SM, Jayamaha CJS. Optimization of isolation of BK viral DNA from renal transplant patients and VP1 gene- based genotyping. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 37
Is total serum nitrite and nitrate (NOx) level in dengue patients a potential prognostic marker of dengue haemorrhagic fever?
Maheshi Sasika Mapalagamage, Shiroma Mangalika Handunnetti, Gayani Premawansa, Sharmila Thillainathan, Geekiyanage Tharanga Geethapriya Fernando, Karunayokiny Kanapathippillai, Rajitha Wickremeshinghe, Aruna Dharshan de Silva, Sunil Premawansa. Is total serum nitrite and nitrate (NOx) level in dengue patients a potential prognostic marker of dengue haemorrhagic fever? Disease Markers, 2018; Article ID: 5328681, doi.org/10.1155/2018/5328681
Is Catalase KatE activity a survival mechanism of Leptospira interrogans?
Barupala NP, Fernando N, Handunnetti SM and Premawansa S. Is Catalase KatE activity a survival mechanism of Leptospira interrogans? Proceeding of the 38th Annual Sessions of the IOB-SL, 2018; pp: 30
In vivo and in vitro studies on anti-inflammatory potential of Alpinia calcarata Rosc. Rhizome and leaf essential oils
Chandrakanthan M, Kathigamanthar S, Maurya AK, Pal A, Handunnetti SM, Premakumara GAS. In vivo and in vitro studies on anti-inflammatory potential of Alpinia calcarata Rosc. Rhizome and leaf essential oils. Asian Symposium on Medicinal Plants, Spices and Other Natural Products XVI, Colombo, Sri Lanka, 12-14th December 2018, pp 92
In vitro study on the effect of antimicrobial agents on inflammatory cytokine response in leptospirosis. (OP11)
Sam G, Fernando N, Rajapakse S, Handunnetti SM In vitro study on the effect of antimicrobial agents on inflammatory cytokine response in leptospirosis. (OP11). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 25
In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants
Perera, HDSM, Samarasekara R, Handunnetti S, Weerasena OVDS, Weeratunga HD, Jabeen A, Chaudhary MI. In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants. BMC Complementary and Alternative Medicine. 2018; 18: 271
Immuno-proteomic characterization of Vespa affinis (Lesser Banded Hornet) venom and its cross-reactivity with the venom of Vespula vulgaris
Gunasekara DLPE, Handunnetti SM, Premawansa S, Dasanayake WMDK, Karunatilake C, Ratnayake IP, Seneviratne SL, Dias RKS, Premakumara GAS, De Silva R. Immuno-proteomic characterization of Vespa affinis (Lesser Banded Hornet) venom and its cross-reactivity with the venom of Vespula vulgaris. Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 33
IgM and IgG response against a specific protein antigen in rats orally fed with a herbal formulation. (OP10)
Ranaweera BVLR, Abeysekera A, Weerasena OVDSJ, Handunnetti SM. IgM and IgG response against a specific protein antigen in rats orally fed with a herbal formulation. (OP10). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 24
High levels of Angiopoietin 2 and Angiopoietin 2/1 at critical stage of DHF patients and their association with clinical and biochemical parameters. (OP09)
Mapalagamage MS, Handunnetti SM, Wickremasinghe R, Premawansa G, Thillainathan S, Fernando T, Khanapathypillai K, De Silva AD, Premawansa S. High levels of Angiopoietin 2 and Angiopoietin 2/1 at critical stage of DHF patients and their association with clinical and biochemical parameters. (OP09). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 23
Direct degranulation of rat mast cells (RBL-2H3) and increase in generation of superoxide anion (O2-) induced by aqueous extract of Acacia pollen and Apis dorsata venom. (OP06)
Ishaka GKD, Gangani PD, Rukshala BAD, Gunasekara DLPE, Fernando N, Handunnetti SM. Direct degranulation of rat mast cells (RBL-2H3) and increase in generation of superoxide anion (O2-) induced by aqueous extract of Acacia pollen and Apis dorsata venom. (OP06). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 20
Development of a colourimetric reverse transcription loop-mediated isothermal amplification assay for the rapid detection of different serotypes of the dengue virus
Ariyaratne MHJD, Nguyen A, De Silva AD, Fernando N, Weerasena OVDSJ, Handunnetti SM. Development of a colourimetric reverse transcription loop-mediated isothermal amplification assay for the rapid detection of different serotypes of the dengue virus. (OP12). Proceeding of the 10th Annual Scientific Sessions of IBMBB, University of Colombo. 21st November 2018, pp: 26